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Integra Bioscience Culture Flask CELLine
N1
The cell culture flask CELLine is a disposable bioreactor based on two-compartment technology. It allows to cultivate cells at highest densities (10
7
-10
8
cells/ml) and to express proteins like
monoclonal antibodies at very high concentrations.
The bioreactor CELLine is separated into a medium and a cell compartment by means of a 10 kDa semi-permeable membrane. This membrane allows a continuous diffusion of nutrients into the
cell compartment with a concurrent removal of any inhibitory waste. The individual accessibility of the compartments allows supplying cells with fresh medium without mechanically interfering
with the culture. Optimal oxygen supply and control of carbon dioxide levels is ensured by a silicone membrane which forms the cell compartment base.
CELLine classic (CL) is ideal for laboratory scale applications using suspension cells or adherent cells in combination with microcarriers. The unit is optimised for cultivation of hybridomas and
many other cell types (e.g. CHO, NSO, SF cells).
CELLine adhere (AD) is specifically adapted to allow growth of anchorage-dependent cells (e.g. HEK, BHK, CHO cells). The bioreactor contains a woven, polyethylene terephtalate (PET) matrix
in the cell compartment providing an ideal surface for cell attachement.
Technical Specifications
CELLine CL350
CELLine CL1000
CELLine AD1000
Size: L x W x H, mm
190 x 95 x 62
275 x 120 x 80
275 x 120 x 80
Weight (g)
185
334
336
Medium compartment cap
28 mm vented (0.2 µm), green polypropylene
cap with polypropylene liner
38 mm vented (0.2 µm), white polypropylene
cap with polypropylene liner
38 mm vented (0.2 µm), black polypropylene
cap with polypropylene liner
Cell compartment cap
24 mm polypropylene cap with polyethylene liner 28 mm polypropylene cap with polyethylene liner 28 mm polypropylene cap with polyethylene liner
Cell compartment inlay
None
None
PET matrix inlay
Microscopic viewing (inverted microscope) center window requires objectice working distances
of 2.5 mm
center window requires objectice working distances
of 2.5 mm
limited visibility due to inlay matrix
Vertical and horizontal volume markings,mL 50 - 350
100 - 1000
100 - 1000
Code
Description
Pack
I90010
Disposable Two-Compartment Bioreactor for suspension cells, 350mL media volume, 5mL culture volume
5
I90005
Disposable Two-Compartment Bioreactor for suspension cells, 1L media volume, 15mL culture volume
3
I90025
Disposable Two-Compartment Bioreactor with matrix inlay for anchorage-dependent cells, 1L media volume, 15mL culture volume
3
TISSUE CUL
TURE
, EQUIPMENT
TISSUE CULTURE, EQUIPMENT
954